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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 2: Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Topic 3: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 4: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 5: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 6: Blood Banking | 15–20% | - Transfusion practice and testing - Blood products and components - Blood group systems and immunology |
| Topic 7: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Haemophilus influenzae is both X and V factor dependent for growth, and is the most common cause of obstructive laryngitis and epiglottitis. H. parainfluenzae is only V-factor dependent and causes only mild pharyngitis, rarely obstructive in nature. H. aphrophilus can be part of the normal upper respiratory flora in humans, does not cause pharyngitis, and is not X or V-factor dependent for growth. Although H. haemolyticus is both X and V-factor dependent, it is beta hemolytic on blood agar and is generally considered non pathogenic.
Acute obstructive epiglottitis, both in adults and children, is caused by the bacterial species shown in this split screen photograph. The colonies recovered on chocolate agar (upper frame) required both hematin and NAD, (as shown by colony growth only between the X and V strips in the lower frame). The most likely identification is:
A) Haemophilus aphrophilus
B) Haemophilus parainfluenzae
C) Haemophilus haemolyticus
D) Haemophilus influenzae
2. What additional fraction would be seen if plasma rather than serum was subjected to electrophoresis:
A) Alpha-1 antitrypsin
B) Fibrinogen
C) Alpha-2 macroglobulin
D) Gamma globulins
3. A test with high specificity accurately detects the absence of disease. The more specific a test is, the fewer false-positive results will occur. A test with high sensitivity accurately identifies the presence of disease. The more sensitive a test, the fewer false-negative results it produces. In the case stated in this question, the immunoassay has high specificity, so it has few false-positives and will accurately detect those individuals who do not have the disease or condition that is being tested for. However, the test has low sensitivity, so it may not identify all individuals who actually have the disease; it may produce many false-negative results.
The accuracy of an immunoassay is its ability to discriminate between results that are true positives and results that are true negatives. Two parameters of test accuracy are specificity and sensitivity. Which of these statements apply to an immunoassay with high specificity, but low sensitivity?
A) Has few false-negatives
B) Has few false-positives
C) Accurately identifies the presence of disease
D) Accurately identifies the absence of disease
4. Which of the following fields is NOT an aspect of clinical microbiology?
A) virology
B) pathology
C) bacteriology
D) parasitology
5. Healthcare workers that worked closely with patient specimens were at an increased risk of contracting which viral infection before a vaccine was developed?
A) HIV
B) Hepatitis C
C) Hepatitis B
D) Hepatitis A
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: D | Question # 3 Answer: B,D | Question # 4 Answer: B | Question # 5 Answer: C |


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